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alexa fluor 488 rat anti mouse cd112  (R&D Systems)


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    Structured Review

    R&D Systems alexa fluor 488 rat anti mouse cd112
    Alexa Fluor 488 Rat Anti Mouse Cd112, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+mouse+cd112+alexa+fluor+488/Mouse+Nectin-2%2FCD112+Alexa+Fluor%C2%AE+488-conjugated+Antibody/pm38474388-73-47-54
    Average 92 stars, based on 2 article reviews
    alexa fluor 488 rat anti mouse cd112 - by Bioz Stars, 2026-11
    92/100 stars

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    Related Articles

    Binding Assay:

    Article Title: CD112 Regulates Angiogenesis and T Cell Entry into the Spleen.
    Article Snippet: In vitro cultured MS-1, imLECs and primary LN LECs were washed with phosphatebuffered saline (PBS) and detached with Accutase (Sigma-Aldrich). .. Unspecific FcRγ binding was blocked with anti-mouse CD16/32 antibody (Biolegend, Luzern, Switzerland), prior to staining with the following primary antibodies and corresponding isotype controls: 20 μg/mL mouse anti-human CD112 (clone: R2.525, Santa Cruz Biotechnology, Heidelberg, Germany) (isotype: Mouse IgG1; clone MG1-45, Biolegend), 2.5 μg/mL rat anti-mouse CD112-Alexa Fluor 488 (clone: 829038, R&D systems, Abingdon, UK) (isotype: Alexa Fluor Rat IgG2a; clone RTK2758, Biolegend). .. Alexa Fluor 488-conjugated secondary antibodies (Caltag Laboratories, Little Balmer, UK) were used for detection in case of uncoupled primary antibodies.

    Article Title: CD112 Regulates Angiogenesis and T Cell Entry into the Spleen
    Article Snippet: In vitro cultured MS-1, imLECs and primary LN LECs were washed with phosphate-buffered saline (PBS) and detached with Accutase (Sigma-Aldrich). .. Unspecific FcRγ binding was blocked with anti-mouse CD16/32 antibody (Biolegend, Luzern, Switzerland), prior to staining with the following primary antibodies and corresponding isotype controls: 20 μg/mL mouse anti-human CD112 (clone: R2.525, Santa Cruz Biotechnology, Heidelberg, Germany) (isotype: Mouse IgG1; clone MG1-45, Biolegend), 2.5 μg/mL rat anti-mouse CD112-Alexa Fluor 488 (clone: 829038, R&D systems, Abingdon, UK) (isotype: Alexa Fluor Rat IgG2a; clone RTK2758, Biolegend). .. Alexa Fluor 488-conjugated secondary antibodies (Caltag Laboratories, Little Balmer, UK) were used for detection in case of uncoupled primary antibodies.

    Staining:

    Article Title: CD112 Regulates Angiogenesis and T Cell Entry into the Spleen.
    Article Snippet: In vitro cultured MS-1, imLECs and primary LN LECs were washed with phosphatebuffered saline (PBS) and detached with Accutase (Sigma-Aldrich). .. Unspecific FcRγ binding was blocked with anti-mouse CD16/32 antibody (Biolegend, Luzern, Switzerland), prior to staining with the following primary antibodies and corresponding isotype controls: 20 μg/mL mouse anti-human CD112 (clone: R2.525, Santa Cruz Biotechnology, Heidelberg, Germany) (isotype: Mouse IgG1; clone MG1-45, Biolegend), 2.5 μg/mL rat anti-mouse CD112-Alexa Fluor 488 (clone: 829038, R&D systems, Abingdon, UK) (isotype: Alexa Fluor Rat IgG2a; clone RTK2758, Biolegend). .. Alexa Fluor 488-conjugated secondary antibodies (Caltag Laboratories, Little Balmer, UK) were used for detection in case of uncoupled primary antibodies.

    Article Title: CD112 Regulates Angiogenesis and T Cell Entry into the Spleen
    Article Snippet: In vitro cultured MS-1, imLECs and primary LN LECs were washed with phosphate-buffered saline (PBS) and detached with Accutase (Sigma-Aldrich). .. Unspecific FcRγ binding was blocked with anti-mouse CD16/32 antibody (Biolegend, Luzern, Switzerland), prior to staining with the following primary antibodies and corresponding isotype controls: 20 μg/mL mouse anti-human CD112 (clone: R2.525, Santa Cruz Biotechnology, Heidelberg, Germany) (isotype: Mouse IgG1; clone MG1-45, Biolegend), 2.5 μg/mL rat anti-mouse CD112-Alexa Fluor 488 (clone: 829038, R&D systems, Abingdon, UK) (isotype: Alexa Fluor Rat IgG2a; clone RTK2758, Biolegend). .. Alexa Fluor 488-conjugated secondary antibodies (Caltag Laboratories, Little Balmer, UK) were used for detection in case of uncoupled primary antibodies.

    Article Title: CD112 Regulates Angiogenesis and T Cell Entry into the Spleen.
    Article Snippet: Single-cell suspensions of mouse tissues (ears, LNs and spleens) were obtained by enzymatic digestion with 4 mg/mL collagenase IV (Invitrogen, Basel, Switzerland) for 45 min at 37 ◦C and subsequently passed through a 40 μm cell strainer (Invitrogen), as described previously [20]. .. Cell suspensions were stained with rat anti-mouse CD31-APC, rat anti-mouse CD45-PerCP, hamster anti-mouse podoplanin-PE/Cy7, rat anti-mouse mucosal vascular addressin cell-adhesion molecule 1 (MadCAM-1)-PE, rat anti-mouse PNAd-Biotin plus Streptavidin APC/Cy7, rat anti-mouse ICAM-1-FITC (all from BioLegend) and rat anti-mouse CD112-Alexa Fluor 488 (R&D system) or corresponding isotype controls. .. To quantify MadCAM-1+CD31+ BECs, spleens were digested with 4 mg/mL collagenase IV (Invitrogen) for 45 min at 37 ◦C and subsequently passed through a 40 μm cell strainer (Invitrogen).

    Article Title: CD112 Regulates Angiogenesis and T Cell Entry into the Spleen
    Article Snippet: Single-cell suspensions of mouse tissues (ears, LNs and spleens) were obtained by enzymatic digestion with 4 mg/mL collagenase IV (Invitrogen, Basel, Switzerland) for 45 min at 37 °C and subsequently passed through a 40 μm cell strainer (Invitrogen), as described previously [ ]. .. Cell suspensions were stained with rat anti-mouse CD31-APC, rat anti-mouse CD45-PerCP, hamster anti-mouse podoplanin-PE/Cy7, rat anti-mouse mucosal vascular addressin cell-adhesion molecule 1 (MadCAM-1)-PE, rat anti-mouse PNAd-Biotin plus Streptavidin APC/Cy7, rat anti-mouse ICAM-1-FITC (all from BioLegend) and rat anti-mouse CD112-Alexa Fluor 488 (R&D system) or corresponding isotype controls. .. To quantify MadCAM-1 + CD31 + BECs, spleens were digested with 4 mg/mL collagenase IV (Invitrogen) for 45 min at 37 °C and subsequently passed through a 40 μm cell strainer (Invitrogen).



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